Our Technology
FLAP&CAP + FLAP&ZAP
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FLAP&CAP
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FLAP&CAP uses flap endonuclease to excise predetermined regions for capture and extensive enrichment, allowing users to concentrate on regions of critical importance. This process accurately identifies key genetic and epigenetic markers through advanced sequencing technologies, offering personalized insights.
Precision Epigenetics for a Healthier Future
At GenesX, we move beyond a 4-base genome to provide comprehensive insights into both genetics and epigenetics. Our patented FLAP&CAP and FLAP&ZAP technologies deliver precise, multiomic data that empowers researchers and clinicians to unlock the full story behind gene regulation and disease.
Our Patented Technologies
Our ‘one-pot’ solution seamlessly captures genetic and epigenetic information from single DNA strands using either short- or long-read next-generation sequencing pipelines.
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FLAP&CAP: Excises, captures, and enriches targeted DNA regions—preserving DNA integrity and pinpointing key genetic and epigenetic markers.
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FLAP&ZAP: Excises and zaps/degrades targeted DNA regions, eliminating unwanted or contaminating sequences that may interfere with downstream analyses.
Both methods provide unparalleled accuracy and efficiency, supporting early cancer detection, functional genomics, and personalized diagnostics.
How It Works
Our patented FENGC platform uses oligonucleotides engineered to form a “flap” structure at both ends of target genomic regions.
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With FLAP&CAP, the flap enables precise cleavage of regions of interest that are enriched for next-generation sequencing, yielding high on-target read percentages with minimal sample input.
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With FLAP&ZAP, the flap facilitates targeted cutting and degradation, effectively “zapping” unwanted segments that contaminate/interfere with downstream analyses.
Both approaches provide cost-effective, high-throughput solutions for modern genetic and epigenetic research.
The Science Behind Our Innovations
FLAP&CAP – Advanced Sequence Capture
FLAP&CAP is an advanced iteration of the flap-enabled next-generation capture (FENGC) technology, designed for multiplexed, targeted genotypic and/or epigenetic analysis. This method involves engineering synthetic oligonucleotides to hybridize with sequences flanking specific genomic regions, forming “FLAP” structures at both ends of a cohort of target segments, preparing them for isolation. Flap endonuclease in the reaction precisely cleaves the DNA flaps at defined junctions, exposing the ends of all target regions. Following excision, DNA ligase is introduced to capture (CAP) target regions by ligating them to universal, exonuclease-resistant oligonucleotides. Subsequent exonuclease digestion and PCR dramatically enrich and amplify the target regions while preserving the continuity of the excised DNA molecules.
The resulting FLAP&CAP approach enables genetic analysis alone or in combination with two epigenetic readouts (DNA methylation with or without chromatin accessibility) and consistently yields high on-target read percentages for numerous targets with minimal starting material. The highly specific, reproducible capture and single-molecule sequencing output are ideal for revealing heterogeneity in a wide range of diagnostic and experimental epigenetic and genetic applications.
FLAP&ZAP – Precision Removal of Interfering Sequences
FLAP&ZAP builds on the FENGC platform by incorporating targeted deletion. In this iteration, oligonucleotides are engineered to form FLAP structures at predetermined DNA regions; however, instead of capturing the targeted sequence(s) after cleavage with flap endonuclease, the process is directed toward complete removal or “ZAP” of the targeted region(s). By combining precision cleavage and degradation, FLAP&ZAP achieves high specificity and minimal off-target effects. The highly selective degradation is particularly useful for elimination of unwanted sequences that may interfere with downstream analyses.
Explore our technology, reveal deeper insights, and join us as we redefine genomic and epigenomic analysis.